Protein Tyrosine Phosphatase Type IVA, Member 3 (PTP4A3) antibody

Antigen: Protein Tyrosine Phosphatase Type IVA, Member 3 (PTP4A3)

Synonyms: PRL3, PRL-3, PRL-R, zgc:77109, wu:fc54b05, wu:fv52d11, prl-3, PTP4A3, MGC155222

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184682

Additional Information

Alternative name: PRL3 / PTP4A3

Gene ID: 11156, 19245 (mouse)

Immunogen: NRPAPVEVSYKHMR-C

Format: Affinity-purified

Description: All names and symbols: PRL3, PTP4A3, protein tyrosine phosphatase type IVA, member 3, PRL-3, PRL-R, potentially prenylated protein tyrosine phosphatase. Official Symbol: PTP4A3. RefSeq number(s): NP_009010.2, NP_116000.1. Gene ontology terms: prenylated protein tyrosine phosphatase activity

Specificity: This antibody is expected to recognise both Human isoforms of this protein according to NP_009010.2 and NP_116000.1. Human, Mouse 148 aa, 173 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 22kDakDa band observed in Human Pancreas lysates (calculated MW of 19.5Da according to NP_116000.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184682 staining (0.03µg/ml) of Human Pancreas lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Saha, Bardelli, Buckhaults et al.: "A phosphatase associated with metastasis of colorectal cancer." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1343-6, 2001 (PubMed).