Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2) antibody

Antigen: Ras-Related C3 Botulinum Toxin Substrate 2 (Rho Family, Small GTP Binding Protein Rac2) (RAC2)

Synonyms: Gx, EN-7, HSPC022, AI323801, AI452260, MGC105983, rac2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184689

Additional Information

Alternative name: RAC2

Gene ID: 5880

Immunogen: PQPTRQQKRACSLL

Format: Affinity-purified

Description: All names and symbols: RAC2, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2), EN-7, Ras-related C3 botulinum toxin substrate 2, rho family, small GTP binding protein Rac2, Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2). Official Symbol: RAC2. RefSeq number(s): NP_002863.1. Gene ontology terms: Rho small monomeric GTPase activity, small GTPase mediated signal transduction, signal transduction

Specificity: Human, Mouse, Rat 192 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 22kDa band observed in lysates of Human T cell line MOLT4 (calculated MW of 21.4kDa according to NP_002863.1). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Tonsil, stains most cells in the mantle zone and interfollicular area. In the germinal centres the majority of cells are negative. Recommended concentration 0.3ug/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184689 staining (2µg/ml) of MOLT-4 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184689 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining in the interfollicular area and mantle zone. B) Positive cells in the interfollicular area.

Publications

Publications: Reibel, Dorseuil, Stancou et al.: "A hemopoietic specific gene encoding a small GTP binding protein is overexpressed during T cell activation." in: Biochemical and biophysical research communications, Vol. 175, Issue 2, pp. 451-8, 1991 (PubMed).