Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RACGAP1 antibody (C-Term)

Verified RACGAP1 Reactivity: Human WB, IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN184690
  • Target See all RACGAP1 Antibodies
    RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))
    Binding Specificity
    • 20
    • 15
    • 13
    • 8
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 73
    • 32
    • 26
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    Human
    Host
    • 83
    • 4
    • 2
    Goat
    Clonality
    • 84
    • 5
    Polyclonal
    Conjugate
    • 47
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This RACGAP1 antibody is un-conjugated
    Application
    • 81
    • 40
    • 34
    • 32
    • 26
    • 26
    • 15
    • 14
    • 10
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    RACGAP1 / MgcRacGAP
    Sequence
    GRQGNFFASP MLK
    Specificity
    This antibody is expected to recognize all reported isoforms (NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_0011
    Cross-Reactivity
    Cow, Dog, Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1 μg/mL.

    Western Blot: Approx 75 kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0 kDa according to NP_037409.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room te

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))
    Alternative Name
    RACGAP1 (RACGAP1 Products)
    Synonyms
    CYK4 antibody, HsCYK-4 antibody, ID-GAP antibody, MgcRacGAP antibody, cyk-4 antibody, id-gap antibody, mgcracgap antibody, RACGAP1 antibody, MGC121578 antibody, Gap antibody, RasGAP antibody, Rasa antibody, GAPX antibody, AI227039 antibody, AI327394 antibody, Band25 antibody, GTPase antibody, gtl11 antibody, mKIAA1478 antibody, wu:fb92c08 antibody, zgc:77839 antibody, Rac GTPase activating protein 1 antibody, rac GTPase-activating protein 1 antibody, Rac GTPase activating protein 1 L homeolog antibody, RAS p21 protein activator 1 antibody, Rac GTPase-activating protein 1 antibody, si:ch1073-416j23.1 antibody, RACGAP1 antibody, racgap1 antibody, racgap1.L antibody, racgap1.2.L antibody, racgap1.2 antibody, Rasa1 antibody, Racgap1 antibody, si:ch1073-416j23.1 antibody
    Background
    RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4
    Gene ID
    29127
    NCBI Accession
    NP_037409, NP_001306934, NP_001306935, NP_001306936
    Pathways
    Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor
You are here:
Support