Polo-Like Kinase 4 (PLK4) antibody

Antigen: Polo-Like Kinase 4 (PLK4)

Synonyms: SAK, STK18

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184701

Additional Information

Alternative name: Sak / STK18 / PLK4

Gene ID: 10733

Immunogen: ATCIGEKIEDFKVGN

Format: Affinity-purified

Description: All names and symbols: PLK4, Sak, polo-like kinase 4 (Drosophila), serine/threonine kinase 18, SAK, Snk Akin Kinase, polo-like kinase 4, protein-serine/threonine kinase. Official Symbol: PLK4. RefSeq number(s): NP_055079.2. Gene ontology terms: protein serine/threonine kinase activity, protein amino acid phosphorylation, regulation of cell cycle

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 95kDa band observed in Human Placenta lysates (calculated MW of 109kDa according to NP_055079.2). Recommended concentration: 0.1-0.3µg/ml. An additional faint band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184701 (0.1µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hudson, Chen, Fode et al.: "Sak kinase gene structure and transcriptional regulation." in: Gene, Vol. 241, Issue 1, pp. 65-73, 2000 (PubMed).