VAV2 antibody (Internal Region)

Catalog No. ABIN184702

Product Details anti-VAV2 Antibody

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This VAV2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: VAV2

Sequence: ETEAKYYRTL EDIEC

Specificity: This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence ETEAKYYRTLEDIEC, from the internal region of the protein sequence according to NP_001127870.1, NP_003362.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 100 kDa band observed in 293 lysate (predicted MW of 100 kDa according to NP_003362). Recommended for use at 1-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for VAV2

Target: VAV2 (Vav 2 Guanine Nucleotide Exchange Factor (VAV2))

Alternative Name: VAV2 (VAV2 Products)

Background: VAV2, vav 2 oncogene, Oncogene VAV2, vav 2 guanine nucleotide exchange factor, Protein vav-2, oncogene VAV2

Gene ID: 7410, 22325

NCBI Accession: NP_001127870, NP_003362

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway