You are viewing an incomplete version of our website. Please click to reload the website as full version.

DKK3 (C-Term) antibody

Details for Product No. ABIN184709, Supplier: Log in to see
Antigen
  • AW061014
  • C87148
  • REIC
  • RIG
  • Reic
  • p29
Epitope
C-Term
35
23
14
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
124
39
29
5
1
1
1
Host
Goat
93
25
18
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
7
6
6
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
90
83
62
14
12
11
4
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Peptide with sequence C-AAAALLGGEEI, from the C Terminus of the protein sequence according to NP_037385.2, NP_056965.3, NP_001018067.1.
Blocking Peptide Blocking peptide for this product available: ABIN368779
Specificity NP_037385.2, NP_056965.3 and NP_001018067.1 are varients of the same protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DKK3 / REIC
Background Synonyms: DKK3, REIC, dickkopf homolog 3 (Xenopus laevis), DKK-3, RIG-like 5-6, RIG-like 7-1, dickkopf 3, Dickkopf gene 3, dickkopf (Xenopus laevis) homolog 3, dickkopf homolog 3, RIG, regulated in glioma
Gene ID 27122
NCBI Accession NP_001018067, NP_037385, NP_056965
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 70 kDa band observed in lysates of cell line HEK293 (calculated MW of 38.4 kDa according to NP_037385.2, NP_056965.3 and NP_001018067.1). The observed molecular weight corresponds to earlier findings in literature (Krupnik et al, Gene. 1999 Oct 1,238(2):301-13., PMID: 10570958 ). Recommended concentration: 0.03-0.1 µg/mL.
IHC: In paraffin embedded Human Brain Cortex shows vesiculate staining in cytoplasm of neuronal cell bodies. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-DKK3 (C-Term) antibody (ABIN184709) ABIN184709 staining (2µg/ml) of HEK293 lysate (RIPA buffer, 30µg total protein per la...
Background publications Krupnik, Sharp, Jiang et al.: "Functional and structural diversity of the human Dickkopf gene family." in: Gene, Vol. 238, Issue 2, pp. 301-13, 1999 (PubMed).