CYTIP antibody (N-Term)

Catalog No. ABIN184711

Product Details anti-CYTIP Antibody

Target: CYTIP (Cytohesin 1 Interacting Protein (CYTIP))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CYTIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: CYBR / PSCDBP

Sequence: SLQRLLQHSS NGN

Cross-Reactivity: Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence SLQRLLQHSSNGN-C, from the N Terminus of the protein sequence according to NP_004279.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 38 kDa band observed in lysates of cell line Jurkat and approx 40 kDa in ysates of cell line MOLT4 (calculated MW of 40 kDa according to NP_004279.3). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of A431 and U251 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYTIP

Target: CYTIP (Cytohesin 1 Interacting Protein (CYTIP))

Alternative Name: CYTIP (CYTIP Products)

Background: CYTIP, PSCDBP, CYBR, pleckstrin homology, Sec7 and coiled/coil domains, binding protein, HE, B3-1, CASP, cytohesin binding protein HE, cytohesin-1 interacting protein, pleckstrin homology, Sec7, and coiled-coil domains protein-binding protein, cytohesin 1

Gene ID: 9595

NCBI Accession: NP_004279