Wingless-Type MMTV Integration Site Family, Member 4 (WNT4) antibody

Antigen: Wingless-Type MMTV Integration Site Family, Member 4 (WNT4)

Synonyms: WNT-4, SERKAL

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184715

Additional Information

Alternative name: WNT4

Gene ID: 54361, 22417 (mouse)

Immunogen: C-SNWLYLAKLSSVGS

Format: Affinity-purified

Description: All names and symbols: WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor. Official Symbol: WNT4. RefSeq number(s): NP_110388.2. Gene ontology terms: cell-cell signaling, development, extracellular matrix structural constituent, frizzled-2 signaling pathway, signal transducer activity

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 35kDa band observed in lysates of Human Testes and Ovary (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Organogenesis, Reproduction, Wnt

Restrictions: For Research Use only

Images

ABIN184715 staining (0.03 ug/ml) of a) Human Ovary and b) Human Testis (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Huguet, McMahon, McMahon et al.: "Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue." in: Cancer research, Vol. 54, Issue 10, pp. 2615-21, 1994 (PubMed).