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AXIN1 antibody (Axin 1) (C-Term)

Details for Product anti-AXIN1 Antibody No. ABIN184720, Supplier: Log in to see
Antigen
  • AI316800
  • Axin
  • AXIN
  • axin
  • AXIN1
  • AXIN1-RGS
  • Fu
  • fused
  • Kb
  • Ki
  • kinky
  • knobbly
  • LOC100226467
  • PPP1R49
  • wu:fi22e03
  • wu:fj14c08
  • wu:fk11b03
  • xAxin
  • zgc:109872
Alternatives
anti-Human AXIN1 antibody for ELISA
Epitope
C-Term
47
16
15
15
13
10
4
4
3
3
2
2
2
2
2
1
1
Reactivity
Human
111
35
27
5
4
2
1
1
1
1
1
Host
Goat
86
18
14
Clonality
Polyclonal
Conjugate
This AXIN1 antibody is un-conjugated
8
8
6
5
5
5
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
86
63
30
15
11
10
5
4
3
1
Supplier
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Immunogen Peptide with sequence C-FEEKIIGKVEKVD, from the C Terminus of the protein sequence according to NP_003493.1, NP_851393.1.
Blocking Peptide Blocking peptide for this product available: ABIN370301
Specificity This antibody is expected to recognise both reported isoforms.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name AXIN1 (AXIN1 Antibody Abstract)
Background Synonyms: AXIN1, axin 1, AXIN, LA16c-314G4.3, MGC52315, axis inhibition protein 1, axis inhibitor 1, fused, mouse, homolog of
Gene ID 8312, 12005, 79257
NCBI Accession NP_003493, NP_851393
Pathways WNT Signaling
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave bands at approx 150 kDa and 22-24 kDa in Human Brain (Hippocampus) lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6 kDa according to NP_003493.1 and 91.7 kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Adrenal Gland shows membranous and fibrous staniing in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Zeng, Fagotto, Zhang, Hsu, Vasicek, Perry, Lee, Tilghman, Gumbiner, Costantini: "The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation." in: Cell, Vol. 90, Issue 1, pp. 181-92, 1997