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Interferon Regulatory Factor 6 (IRF6) (C-Term) antibody

Details for Product No. ABIN184730
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Antigen
Synonyms LPS, OFC6, PIT, PPS, PPS1, VWS, VWS1, mirf6, AI876454, E230028I05Rik, zgc:63500, xIRF-6, IRF6, lps, pit, pps, vws, ofc6, DKFZp469E012
Epitope
C-Term
(6), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(50), (23), (18), (6), (5), (5), (4), (4), (4), (1), (1), (1)
Host
Goat
(33), (13), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(41), (14), (11), (10), (10), (8), (4), (4), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Availability Available
Catalog No. ABIN184730
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-TPSMQLPPALPPQ, from the C Terminus of the protein sequence according to NP_006138.1.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name IRF6
Background Synonyms: IRF6, interferon regulatory factor 6, LPS, PIT, PPS, VWS, OFC6
Gene ID 3664, 54139
NCBI Accession NP_006138.1
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60 kDa band observed in Human Ovary lysates (calculated MW of 53.1 kDa according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.2-0.6 µg/mL.
IHC: In paraffin embedded Human Kidney shows strong nuclear staining in DCT and glomerulus. Recommended concentration: 2-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Interferon Regulatory Factor 6 (IRF6) (C-Term) antibody ABIN184730 staining (0.2µg/ml) of Human Ovary lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Kondo, Schutte, Richardson et al.: "Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes." in: Nature genetics, Vol. 32, Issue 2, pp. 285-9, 2002 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN184730
374.27 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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