Transmembrane Protein 189 (TMEM189) (221 aa isoform b) antibody

Antigen: Transmembrane Protein 189 (TMEM189)

Synonyms: CIR1, UEV1, CROC1, UBE2V, UEV-1, UEV1A, CROC-1, KUA, UBE2V1, TMEM189-UBE2V1, UBE2V2, CROC-1B, MGC128136, MGC123326, im:7136154, Kua, AI840826, RP23-118A2.8, zgc:101801, kua, tm189

Epitope: 221 aa isoform b

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184734

Additional Information

Alternative name: UEV1 / CROC1 (221 aa isoform b)

Gene ID: 7335

Immunogen: AYKFRTHSPEALE-C

Format: Affinity-purified

Description: All names and symbols: UBE2V1, ubiquitin-conjugating enzyme E2 variant 1, CIR1, UEV1, CROC1, UEV-1, UEV1A, CROC-1, DNA-binding protein. Official Symbol: UBE2V1. RefSeq number(s): NP_003340. Gene ontology terms: ubiquitin conjugating enzyme activity, protein polyubiquitination, regulation of DNA repair, regulation of cell cycle

Specificity: This antibody is expected to recognise human isoform b only (NP_003340). Human 221 aa

Application Details

Application Notes: Western blot: Approx 27kDa band seen in K562 lysate. Recommended for use at 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184734 staining (3 ug/ml) of K562 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Rothofsky, Lin: "CROC-1 encodes a protein which mediates transcriptional activation of the human FOS promoter." in: Gene, Vol. 195, Issue 2, pp. 141-9, 1997 (PubMed).