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Iba1 antibody (C-Term)

Verified IBA1 Reactivity: Human, Rat, Mouse WB, IHC, ELISA Host: Goat Polyclonal unconjugated

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

ABIN184738 (1µg/ml) staining of Human Frontal Cortex (A), Rat Brain (B), Mouse (C) Brain and Pig Brain (D) lysates (35µg protein in RIPA buffer).

ABIN184738 (5µg/ml) staining of paraffin embedded Human Lung.

ABIN184738 (1µg/ml) staining of Human Brain (Frontal Cortex (A) Rat (B) Mouse (C) Brain and Swine E) Brain lysates (35µg protein in RIPA buffer).

ABIN184738 Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.

ABIN184738 (1µg/ml) staining of Human (A) and (0.

ABIN184738 (2µg/ml) staining of paraffin embedded Mouse Brain.

ABIN184738 (0.

AIF1 / IBA1 staining of Rat Brain lysate (35 µg protein in RIPA buffer) by ABIN184738 (0.

ABIN184738 (1µg/ml) staining of Human (A) and (0.

ABIN184738 (1µg/ml) staining of Human Frontal Cortex (A) and (0.

ABIN184738 Negative Control showing staining of paraffin embedded Mouse Lung, with no primary antibody.

ABIN184738 Staining of paraffin embedded Mouse Lung.

ABIN184738 (1µg/ml) staining of Human Lymph node (A), Mouse Brain (B) and Rat Brain (C) lysate (35µg protein in RIPA buffer).

ABIN184738 (1 μg/mL) staining of Mouse (A) and (2 μg/mL) Rat (B Brain lysate (35 μg protein in RIPA buffer).

ABIN184738 (6 μg/mL) staining of paraffin embedded Human Placenta.

ABIN184738 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

(ABIN184738) (1 μg/mL) staining of Mouse (A) and Rat (B) Brain lysate (35 μg protein in RIPA buffer).

(ABIN184738) (5 μg/mL) staining of paraffin embedded Human Spleen.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex lysate (35 μg protein in RIPA buffer).

B05419 (0.

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

(ABIN184738) (0.

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Lung.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.

(ABIN184738) (2 μg/mL) staining of Rat (A) and Mouse (B) Brain lysates (35 μg protein in RIPA buffer).

(ABIN184738) (4 μg/mL) staining of paraffin embedded Human Placenta.

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex (A), Mouse (B) and Rat (C) Brain lysates (35 μg protein in RIPA buffer) Detected by chemiluminescence.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

B05419 (0.

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

(ABIN184738) (1 μg/mL) staining of Human Frontal Cortex (A) Mouse Brain (B) and Rat Brain (C) lysate (35 μg protein in RIPA buffer).

(ABIN184738) (6 μg/mL) staining of paraffin embedded Human Spleen.

(ABIN184738) Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

(ABIN184738) (0.

(ABIN184738) (4 μg/mL) staining of paraffin embedded Human Spleen.

41 images

Catalog No. ABIN184738

Datasheet as PDF

Target See all Iba1 (IBA1) Antibodies

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
Binding Specificity
16

16

15

10

9

8

7

7

3

1

1

1

1

1

1

1
C-Term
Reactivity
88

54

47

2

1
Human, Rat, Mouse
Host
77

32

5

1
Goat
Compatible Secondaries
Clonality
78

37
Polyclonal
Conjugate
55

18

13

7

3

2

2

2

2

2

2

2

1

1

1

1

1
This Iba1 antibody is un-conjugated
Application
79

46

42

22

21

14

14

13

12

7

5

4

3

3

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose

AIF1/IBA1 isoform 1 and 3

Sequence

TGPPAKKAIS ELP

Specificity

This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Cross-Reactivity

Human, Mouse, Pig, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3.

Isotype

IgG

Top Product

Discover our top product IBA1 Primary Antibody
anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (C-Term) antibody
IBA1 Reactivity: Human WB, IHC, IHC (p), ICC, IF, FACS, IHC (fro), IHC (ff) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 51-147) antibody
IBA1 Reactivity: Human, Rat, Mouse WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 Reactivity: Human WB, IHC, IP, IHC (p), ICC, IF, FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Rat WB, IHC, IP, ICC Host: Mouse Monoclonal C5 unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (Internal Region) antibody
Verified IBA1 Reactivity: Human IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 1-147) antibody
IBA1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 Reactivity: Mouse WB, IHC, IF Host: Rabbit Monoclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) antibody
IBA1 Reactivity: Human WB, IHC Host: Rabbit Monoclonal unconjugated

anti-Ionized Calcium-binding Adapter Molecule 1 (IBA1) (AA 35-102) antibody
IBA1 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.
Western Blot: Approx. 16-17 kDa band observed in Human Frontal Cortex and in Mouse and Rat Brain lysates (calculated MW of 16.7 kDa according to Human NP_001614.3, 16.9 kDa according to Mouse NP_062340.1 and 16.8 kDa according to Rat NP_058892.1). Recommende
Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Target

Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name

AIF1 (IBA1 Products)

Synonyms

AIF-1 antibody, IBA1 antibody, IRT-1 antibody, IRT1 antibody, AI607846 antibody, D17H6S50E antibody, G1 antibody, Iba1 antibody, Bart1 antibody, iba1 antibody, mrf-1 antibody, fa04b11 antibody, wu:fa04b11 antibody, AIF antibody, AIF1 antibody, aif1 antibody, allograft inflammatory factor 1 antibody, AIF1 antibody, Aif1 antibody, aif1 antibody

Background

AIF1, IBLY409840A1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8

Gene ID

199, 11629, 29427

NCBI Accession

NP_116573, NP_001614

Pathways

Smooth Muscle Cell Migration

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