DBNL antibody (N-Term)

Catalog No. ABIN184747

Product Details anti-DBNL Antibody

Target: DBNL (Drebrin-Like (DBNL))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DBNL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: HIP-55 / SH3P7

Sequence: AANLSRNGPA LQE

Specificity: This antibody is expected to recognise all reported isoforms (NP_054782.2, NP_001014436.1, NP_001116428.1)

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence AANLSRNGPALQE-C, from the N Terminus of the protein sequence according to NP_054782.2, NP_001014436.1, NP_001116428.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 48.3 kDa according to NP_054782.2). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DBNL

Target: DBNL (Drebrin-Like (DBNL))

Alternative Name: DBNL (DBNL Products)

Background: DBNL, HIP-55, SH3P7, src homology 3 domain-containing protein HIP-55, HIP-55 protein, drebrin F, drebrin-like, ABP1

Gene ID: 28988, 13169, 83527

NCBI Accession: NP_054782, NP_001014436, NP_001116428

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization