KIF5B antibody (Kinesin Family Member 5B) (C-Term)

Details for Product anti-KIF5B Antibody No. ABIN184774, Supplier: Log in to see
Antigen
  • Khc
  • KIF5A
  • KINH
  • KNS
  • KNS1
  • UKHC
  • AL022807
  • Khcs
  • Kns1
  • Ukhc
  • khc
  • kinesin heavy chain
  • kinesin family member 5A
  • kinesin family member 5B
  • Khc
  • cgd6_2790
  • NCU09730
  • Tb927.1.1350
  • LACBIDRAFT_305999
  • KIF5A
  • KIF5B
  • Kif5b
  • kif5b
Alternatives
anti-Human KIF5B antibody for ELISA
Epitope
C-Term
27
9
8
4
3
3
2
1
1
1
1
1
1
1
Reactivity
Human, Dog (Canine)
68
33
21
19
16
15
5
4
3
3
3
2
2
2
1
1
1
Host
Goat
38
22
19
Clonality
Polyclonal
Conjugate
This KIF5B antibody is un-conjugated
6
3
3
3
3
3
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
65
40
29
16
13
10
6
5
4
1
1
1
1
Options
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Immunogen Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Blocking Peptide Blocking peptide for this product available: ABIN369683
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Kinesin 1 / UKHC (KIF5B Antibody Abstract)
Background Synonyms: KIF5B, UKHC, kinesin 1 (110-120kD), kinesin family member 5B, KNS, KINH, KNS1, U-KHC, kinesin heavy chain
Gene ID 3799
NCBI Accession NP_004512
Pathways Peptide Hormone Metabolism, Ribonucleoprotein Complex Subunit Organization
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110 kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110 kDa according to NP_004512.1). Recommended concentration: 0.5-1.5 µg/mL.
IHC: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration: 1-2 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat). In paraffin embedded Human Uterus shows strong staining of the epithelium on endometrial glands. Recommended concentration: 2-3 µg/mL. Please note this data was obtained with a previous batch of EB05492 (different goat).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-KIF5B antibody (Kinesin Family Member 5B) (C-Term) (ABIN184774) ABIN184774 staining (0.2µg/ml) of Human Kidney lysate (RIPA buffer, 35µg total protei...
Immunohistochemistry (IHC) image for anti-KIF5B antibody (Kinesin Family Member 5B) (C-Term) (ABIN184774) ABIN184774 (1µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen re...
Product cited in: Astanina, Jacob: "KIF5C, a kinesin motor involved in apical trafficking of MDCK cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 8, pp. 1331-42, 2010 (PubMed).

Gupta, Palmer, Spence, Hudson, Stephens: "Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport." in: Traffic (Copenhagen, Denmark), Vol. 9, Issue 11, pp. 1850-66, 2008 (PubMed).

Background publications Semiz, Park, Nicoloro, Furcinitti, Zhang, Chawla, Leszyk, Czech: "Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules." in: The EMBO journal, Vol. 22, Issue 10, pp. 2387-99, 2003 (PubMed).