RAN Binding Protein 7 (RANBP7) antibody

Antigen: RAN Binding Protein 7 (RANBP7)

Synonyms: Imp7, RANBP7, FLJ14581, MGC138673, imp7, ranbp7, MGC52556, MGC132350

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184802

Additional Information

Alternative name: RANBP7 / Importin 7

Gene ID: 10527

Immunogen: C-SSFNFGGPAPGMN

Format: Affinity-purified

Description: All names and symbols: importin 7, RANBP7, IPO7, FLJ14581, MGC138673, RAN binding protein 7, RAN-binding protein 7. Official Symbol: IPO7. RefSeq number(s): NP_006382.1. Gene ontology terms: transporter, nuclear pore, soluble fraction, signal transduction, RAN protein binding, nucleocytoplasmic transport, small GTPase regulatory/interacting protein

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 120kDa band observed in Human Brain lysates (predicted MW of 119kDa according to NP_006382.1). Recommended for use at 0.1-1µg/ml. Please note that an approx 30kDa band of unknown identity is also consistently observed.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184802 staining (0.1µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Goerlich, Dabrowski, Bischoff et al.: "A novel class of RanGTP binding proteins." in: The Journal of cell biology, Vol. 138, Issue 1, pp. 65-80, 1997 (PubMed).