Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) antibody

Antigen: Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG)

Synonyms: PI3K, PIK3, PI3CG, PI3Kgamma, Pi3k, PIK3CG, wu:fb15d07, MGC157122, pi3k

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184817

Additional Information

Alternative name: PIK3CG

Gene ID: 5294, 30955 (mouse)

Immunogen: C-VLGIKQGEKHSA

Description: All names and symbols: PIK3CG, phosphoinositide-3-kinase, catalytic, gamma polypeptide, PI3K, PIK3, PI3CG, PI3KgammaPI3-kinase, p110-gamma, PTDINS-3-kinase, 1-phosphatidylinositol 3-kinase, phosphatidylinositol 3 kinase gamma, p110 gamma, phosphoinositide-3-kinase gamma catalytic subunit, phosphatidylinositol 3-kinase catalytic 110-kD gamma, phosphatidylinositol 3-kinase, catalytic, gamma polypeptide. Official Symbol: PIK3CG. RefSeq number(s): NP_002640.2. Gene ontology terms: G-protein coupled receptor protein signaling pathway, 1-phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase activity, transferase activity

Specificity: Human, Mouse 1102 aa

Application Details

Application Notes: Western blot: Approx 110-120kDa band seen in Human Heart lysate (Predicted MW of 121kDa according to NP_002640). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Kinases/Phosphatases, Signaling, Cancer

Restrictions: For Research Use only

Images

ABIN184817 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Stoyanov, Volinia, Hanck et al.: "Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase." in: Science (New York, N.Y.), Vol. 269, Issue 5224, pp. 690-3, 1995 (PubMed).