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Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) antibody

Antigen

Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1)
Clonality Polyclonal
Host
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA
1 reference available
Catalog no. ABIN184837
Quantity 100 ug  (Variants)
Price 261.61 $   Plus shipping costs $45.00
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Additional Information
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Alternative name IRE1 / ERN1
Gene ID 2081
Immunogen C-PPEPQPPVTPDAL
Description All names and symbols: ERN1, endoplasmic reticulum to nucleus signalling 1, FLJ30999, IRE1, IRE1P, MGC163277, MGC163279, ER to nucleus signalling 1, inositol-requiring 1, protein kinase/endoribonuclease. Official Symbol: ERN1. RefSeq number(s): NP_001424.3. Gene ontology terms: endoribonuclease activity, ATP binding, protein serine/threonine kinase activity, DNA binding, electron transport, protein amino acid phosphorylation, endoplasmic reticulum membrane, nucleus, transferase activity
Specificity Human 977 aa

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: No signal obtained yet but low background observed in HepG2 lysates at up to 2µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Tirasophon, Welihinda, Kaufman: "A stress response pathway from the endoplasmic reticulum to the nucleus requires a novel bifunctional protein kinase/endoribonuclease (Ire1p) in mammalian cells." in: Genes & development, Vol. 12, Issue 12, pp. 1812-24, 1998 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1)", type "Antibodies"
Hosts Rabbit (66), Mouse (8), Goat (4)
Reactivities Human (74), Mouse (Murine) (50), Rat (Rattus) (47), Dog (Canine) (37), Cow (Bovine) (36), Horse (Equine) (36), Pig (Porcine) (36), Rabbit (36)
Applications Western Blotting (WB) (47), Immunofluorescence (IF) (36), ELISA (28), Immunocytochemistry (ICC) (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (IHC) (2), Flow Cytometry (FACS) (1), Immunoprecipitation (IP) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2)
Epitopes pSer726 (18), C-Term (8), N-Term (7), Internal Region (6), AA 282-433 (2), C-Term,AA 826-855 (1), C-Term,AA 827-857 (1), Middle Region (1), pspecific (1)