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POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody

Details for Product No. ABIN184851, Supplier: Login to see
Antigen
  • BOB1
  • OBF-1
  • OBF1
  • OCAB
  • BOB-1
  • BOB.1
  • Bob-1
  • Bob1
  • OBF.1
  • OCA-B
Epitope
C-Term
45
20
9
9
2
2
1
1
1
Reactivity
Mouse (Murine)
132
43
38
7
5
1
Host
Goat
85
40
15
Clonality
Polyclonal
Conjugate
Un-conjugated
9
9
9
5
5
5
2
2
2
2
2
2
2
2
2
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
98
56
32
29
22
19
3
2
2
2
1
Supplier
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Immunogen Peptide with sequence C-DAYALNHTLSVEGF, from the C Terminus of the protein sequence according to NP_006226.2.
Blocking Peptide Blocking peptide for this product available: ABIN368826
Cross-Reactivity Human, Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BOB1 / OCAB / OBF1 (POU2AF1 Antibody Abstract)
Background Synonyms: BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, associating factor 1, POU class 2 associating factor 1, OCAB
Gene ID 5450, 18985
NCBI Accession NP_006226
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in mouse spleen lysate (Predicted MW of 28 kDa according to NP_006226). Recommended for use at 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody (ABIN184851) ABIN184851 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein ...
Background publications Strubin, Newell, Matthias: "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." in: Cell, Vol. 80, Issue 3, pp. 497-506, 1995 (PubMed).