POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody

Details for Product No. ABIN184851
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Antigen
Synonyms BOB1, OBF-1, OBF1, OCAB, BOB-1, BOB.1, Bob-1, Bob1, OBF.1, OCA-B
Epitope
C-Term
(29), (10), (8), (2), (1), (1), (1)
Reactivity
Mouse (Murine)
(81), (30), (30), (28), (26), (24), (12), (1)
Host
Goat
(68), (12), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(52), (33), (20), (19), (12), (10), (9), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184851
374.27 $
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Immunogen Peptide with sequence C-DAYALNHTLSVEGF, from the C Terminus of the protein sequence according to NP_006226.2.
Cross-Reactivity Human, Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BOB1 / OCAB / OBF1
Background Synonyms: BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, associating factor 1, POU class 2 associating factor 1, OCAB
Gene ID 5450, 18985
NCBI Accession NP_006226
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in mouse spleen lysate (Predicted MW of 28 kDa according to NP_006226). Recommended for use at 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-POU Class 2 Associating Factor 1 (POU2AF1) (C-Term) antibody ABIN184851 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Strubin, Newell, Matthias: "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." in: Cell, Vol. 80, Issue 3, pp. 497-506, 1995 (PubMed).

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