SETDB2 antibody (N-Term)

Catalog No. ABIN184878

Product Details anti-SETDB2 Antibody

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SETDB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: CLLD8 / SETDB2

Sequence: GEKNGDAKTF WME

Specificity: This antibody is expected to recognize both reported isoforms (NP_114121.2, NP_001153780.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence GEKNGDAKTFWME-C, from the N Terminus of the protein sequence according to NP_114121.2, NP_001153780.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 85 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 81.9 kDa according to NP_114121.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SETDB2

Target: SETDB2 (SET Domain, Bifurcated 2 (SETDB2))

Alternative Name: SETDB2 (SETDB2 Products)

Background: SETDB2, CLLD8, SET domain, bifurcated 2, CLLL8 protein, C13orf4, CLLL8, DKFZp586I0123, DKFZp761J1217, KMT1F, chronic lymphocytic leukemia deletion region 8, lysine N-methyltransferase 1F, histone-lysine N-methyltransferase SETDB2

Gene ID: 83852

NCBI Accession: NP_114121, NP_001153780