Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2) antibody
| Antigen |
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| Synonyms |
ALR, MLL4, AAD10, KMT2B, TNRC21, CAGL114, im:7157663 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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| Catalog no. |
ABIN184880 |
| Quantity |
100 ug |
| Price |
374.27 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MLL2
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Gene ID
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8085
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Immunogen
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DSQNLAGEDKDSQ-C
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Description
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All names and symbols: MLL2, myeloid/lymphoid or mixed-lineage leukemia 2, ALR, ALL1-related gene. Official Symbol: MLL2. RefSeq number(s): NP_003473.1. Gene ontology terms: nucleus, oncogenesis, transcription factor, transcription from Pol II promoter, protein ubiquitination, ubiquitin-protein ligase activity
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Specificity
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The immunizing peptide was designed from NP_003473.1. Since then the sequence has changed in the databases and currently differs at two locations in NP_003473.3. Human
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave bands at approx 75kDa and 25kDa in lysates of cell line K562 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 593kDa according to NP_003473.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Restrictions
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For Research Use only
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Prasad, Zhadanov, Sedkov et al.: "Structure and expression pattern of human ALR, a novel gene with strong homology to ALL-1 involved in acute leukemia and to Drosophila trithorax." in: Oncogene, Vol. 15, Issue 5, pp. 549-60, 1997 (PubMed).
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Alternatives for antigen "Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2)", type "Antibodies"
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Hosts
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Rabbit (19), Goat (3), Mouse (1)
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Reactivities
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Human (23), Cow (Bovine) (18), Dog (Canine) (18), Horse (Equine) (18), Mouse (Murine) (18), Pig (Porcine) (18), Rat (Rattus) (18)
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Applications
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Immunofluorescence (IF) (15), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Western Blotting (WB) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (18), N-Term (2), AA 4720-4735 (1)
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Alternatives 
