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UBE2K antibody (C-Term)

Verified UBE2K Reactivity: Human WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN184891
  • Target See all UBE2K Antibodies
    UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
    Binding Specificity
    • 8
    • 8
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 45
    • 21
    • 18
    • 7
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    Human
    Host
    • 43
    • 2
    • 2
    Goat
    Clonality
    • 45
    • 2
    Polyclonal
    Conjugate
    • 24
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This UBE2K antibody is un-conjugated
    Application
    • 36
    • 25
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    HIP2
    Sequence
    SWDVETATEL LLSN
    Specificity
    This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Pig
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-SWDVETATELLLSN, from the C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1.
    Isotype
    IgG
    Top Product
    Discover our top product UBE2K Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration, 1-2 μg/mL.

    Western Blot: Approx 24 kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:2000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))
    Alternative Name
    UBE2K (UBE2K Products)
    Synonyms
    E2-25K antibody, HIP2 antibody, HYPG antibody, LIG antibody, UBC1 antibody, E2-25k antibody, Hip2 antibody, Hypg antibody, Lig antibody, e2-25k antibody, hip2 antibody, hypg antibody, lig antibody, AW492011 antibody, D5Ertd601e antibody, ube2k antibody, zgc:103472 antibody, zgc:110791 antibody, ubiquitin conjugating enzyme E2 K antibody, ubiquitin-conjugating enzyme E2K antibody, ubiquitin conjugating enzyme E2 K S homeolog antibody, ubiquitin-conjugating enzyme E2Kb (UBC1 homolog, yeast) antibody, ubiquitin-conjugating enzyme E2Ka (UBC1 homolog, yeast) antibody, UBE2K antibody, Ube2k antibody, ube2k.S antibody, ube2k antibody, ube2kb antibody, ube2ka antibody
    Background
    UBE2K, HIP2, huntingtin interacting protein 2, LIG, HYPG, ubiquitin-protein ligase, ubiquitin carrier protein, ubiquitin-conjugating enzyme E2-25 KDA, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), DKFZp564C1216, DKFZp686J24237, E2-25K,
    Gene ID
    3093
    NCBI Accession
    NP_005330, NP_001104582, NP_001104583
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