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Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody

Details for Product No. ABIN184897, Supplier: Log in to see
Antigen
  • UBE2T
  • wu:fj86h11
  • zgc:153687
  • HR6B
  • PIG50
  • 2700084L22Rik
  • C80607
  • RGD1310816
  • hspc150
Epitope
C-Term
29
15
6
6
5
3
2
2
1
1
Reactivity
Human
66
12
6
Host
Goat
43
14
9
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
60
38
9
8
1
1
1
Supplier
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Immunogen Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1.
Blocking Peptide Blocking peptide for this product available: ABIN370326
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name HSPC150 / UBE2T (Ube2t Antibody Abstract)
Background Synonyms: UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T
Gene ID 29089
NCBI Accession NP_054895
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 22 kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5 kDa according to NP_054895.1). Recommended concentration: 0.1-0.3 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Machida, Machida, Chen et al.: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).