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Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) (C-Term) antibody

Details for Product No. ABIN184898, Supplier: Log in to see
Antigen
  • NCE2
  • 2510010F15Rik
  • AI851109
  • RGD1307608
  • zgc:77005
Epitope
C-Term
25
14
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
48
26
18
9
9
3
1
1
Host
Goat
39
19
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
42
39
7
5
2
2
Supplier
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Immunogen Peptide with sequence C-RNKVDDYIKRYAR, from the C Terminus of the protein sequence according to NP_542409.1.
Blocking Peptide Blocking peptide for this product available: ABIN369801
Specificity This antibody is expected to recognise isoform 1 (NP_542409.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name UBE2F/NEDD8-conjugating enzyme (UBE2F Antibody Abstract)
Background Synonyms: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme
Gene ID 140739
NCBI Accession NP_542409
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Podust, Brownell, Gladysheva et al.: "A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 9, pp. 4579-84, 2000 (PubMed).