Chromobox Homolog 6/8 (CBX6/8) antibody

Antigen: Chromobox Homolog 6/8 (CBX6/8)

Synonyms: CBX6, MGC143414, cbx6, MGC101049

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN184901

Additional Information

Alternative name: CBX6 and CBX8

Gene ID: 23466, 57

Immunogen: ELSAVGERVFAAE-C

Description: All names and symbols: CBX6, chromobox homolog 6, CBX8, chromobox homolog 8, PC3, RC1, HPC3, polycomb 3, Neuronal pentraxin receptor. Official Symbol: CBX6 / CBX8. RefSeq number(s): NP_055107.3, NP_065700.1

Specificity: This antibody is expected to recognise both the human proteins CBX6 and CBX8, which are almost identical. Human, Mouse, Rat, Dog 412 aa, 389aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 37kDa band in Human, Mouse and Rat Brain lysates at 0.3ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.8kDa according to NP_055107.3 (CBX6) and 43.4 kDa according to NP_065700.1 (CBX8). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Bárdos, Saurin, Tissot et al.: "HPC3 is a new human polycomb orthologue that interacts and associates with RING1 and Bmi1 and has transcriptional repression properties." in: The Journal of biological chemistry, Vol. 275, Issue 37, pp. 28785-92, 2000 (PubMed).