CHFR antibody (C-Term)

Catalog No. ABIN184918

Product Details anti-CHFR Antibody

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: CHFR

Sequence: KFNHICEQTR FKN

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1, NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in human brain lysates at 0.5 μg/mL, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR (CHFR Products)

Synonyms: RNF116 antibody, RNF196 antibody, CHFR antibody, fc43f10 antibody, si:dkey-69h6.7 antibody, wu:fc43f10 antibody, rnf116 antibody, rnf196 antibody, 5730484M20Rik antibody, C230082M18 antibody, checkpoint with forkhead and ring finger domains antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase antibody, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog antibody, CHFR antibody, chfr antibody, Chfr antibody, chfr.L antibody

Background: CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196

Gene ID: 55743, 231600, 288734

NCBI Accession: NP_001154816, NP_001154817, NP_001154818, NP_060693, NP_001154819