NIF3L1 antibody (C-Term)

Catalog No. ABIN184942

Product Details anti-NIF3L1 Antibody

Target: NIF3L1 (NIF3 NGG1 Interacting Factor 3-Like 1 (NIF3L1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NIF3L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: ALS2CR1 / NIF3L1

Sequence: ILSETDRDPL QVV

Specificity: This antibody is expected to recognise both reported isoforms. Variants (NP_001135827.1, NP_068596.2) encode the same isoform.

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ILSETDRDPLQVV, from the C Terminus of the protein sequence according to NP_001129511.1, NP_068596.2

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows strong cytoplasm staining in epithelial and myoepithelial duct cells. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 40 kDa band seen in Human Heart and Human Testis extracts [Predicted MW of approx. 39 kDa according to NP_068596]. Recommended for use at 0.2-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NIF3L1

Target: NIF3L1 (NIF3 NGG1 Interacting Factor 3-Like 1 (NIF3L1))

Alternative Name: NIF3L1 (NIF3L1 Products)

Background: NIF3L1, ALS2CR1, NIF3 NGG1 interacting factor 3-like 1 (S. pombe), CALS-7, MDS015, NIF3 (Ngg1 interacting factor 3, S.pombe homolog)-like 1, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 1, NIF3 NGG1 interacting factor 3-like 1,

Gene ID: 60491, 65102

NCBI Accession: NP_001129511, NP_068596