TRIM55 antibody (C-Term)

Catalog No. ABIN184953

Product Details anti-TRIM55 Antibody

Target: TRIM55 (Tripartite Motif Containing 55 (TRIM55))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRIM55 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: MURF2 / TRIM55

Sequence: HIFSFSWLNS LNE

Specificity: This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HIFSFSWLNSLNE, from the C Terminus of the protein sequence according to NP_908973.1, NP_908974.1, NP_908975.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 10 μg/mL.

Western Blot: Approx 58 kDa band observed in lysates of cell line HeLa (calculated MW of 60.5 kDa according to NP_908973.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TRIM55

Target: TRIM55 (Tripartite Motif Containing 55 (TRIM55))

Alternative Name: TRIM55 (TRIM55 Products)

Synonyms: MURF-2 antibody, RNF29 antibody, muRF2 antibody, trim55 antibody, wu:fb71c01 antibody, zgc:92123 antibody, TRIM55 antibody, im:7145460 antibody, zgc:136833 antibody, D830041C10Rik antibody, Murf2 antibody, Rnf29 antibody, murf-2 antibody, rnf29 antibody, tripartite motif containing 55 antibody, tripartite motif containing 55a antibody, tripartite motif containing 55b antibody, tripartite motif-containing 55 antibody, tripartite motif containing 55 L homeolog antibody, TRIM55 antibody, trim55a antibody, trim55b antibody, Trim55 antibody, trim55.L antibody

Background: TRIM55, RNF29, MURF-2, ring finger protein 29, for muscle specific ring finger 2, tripartite motif-containing 55, muscle specific ring finger 2

Gene ID: 84675, 381485, 365751

NCBI Accession: NP_908973, NP_908974, NP_908975