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TRIM21 antibody (C-Term)

Verified TRIM21 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN184965
  • Target See all TRIM21 Antibodies
    TRIM21 (Tripartite Motif Containing 21 (TRIM21))
    Binding Specificity
    • 15
    • 10
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 55
    • 28
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 50
    • 8
    • 2
    • 1
    Goat
    Clonality
    • 54
    • 7
    Polyclonal
    Conjugate
    • 39
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TRIM21 antibody is un-conjugated
    Application
    • 48
    • 21
    • 13
    • 13
    • 12
    • 9
    • 8
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    SSA1 / Ro52
    Sequence
    CPLNIGSQGS TDY
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence CPLNIGSQGSTDY, from the C Terminus of the protein sequence according to NP_003132.2.
    Isotype
    IgG
    Top Product
    Discover our top product TRIM21 Primary Antibody
  • Application Notes

    Western Blot: In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antib

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    TRIM21 (Tripartite Motif Containing 21 (TRIM21))
    Alternative Name
    TRIM21 (TRIM21 Products)
    Synonyms
    RNF81 antibody, RO52 antibody, SSA antibody, SSA1 antibody, Ro52 antibody, Ssa1 antibody, tripartite motif containing 21 antibody, tripartite motif-containing 21 antibody, TRIM21 antibody, Trim21 antibody
    Background
    TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52 kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome ant
    Gene ID
    6737
    NCBI Accession
    NP_003132
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