NUP50 antibody (C-Term)

Catalog No. ABIN184968

Product Details anti-NUP50 Antibody

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NUP50 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Npap60 / Nup50

Sequence: ELHKILLEKK DA

Specificity: This antibody is expected to recognise both reported isoforms (NP_009103.2, NP_705931.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ELHKILLEKKDA, from the C Terminus of the protein sequence according to NP_009103.2, NP_705931.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-NUP50 antibody (ABIN184968)

Shitashige, Satow, Honda, Ono, Hirohashi, Yamada: "Regulation of Wnt signaling by the nuclear pore complex." in: Gastroenterology, Vol. 134, Issue 7, pp. 1961-71, 1971.e1-4, (2008) (PubMed).

Target Details for NUP50

Target: NUP50 (Nucleoporin 50kDa (NUP50))

Alternative Name: NUP50 (NUP50 Products)

Background: NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nucleoporin 50 kDa, nuclear pore-associated protein 60L, CTA-268H5.7, OTTHUMP00000199530

Gene ID: 10762, 18141

NCBI Accession: NP_009103, NP_705931

Pathways: Tube Formation