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TRIM5 antibody (Tripartite Motif Containing 5) (N-Term)

Details for Product anti-TRIM5 Antibody No. ABIN184983, Supplier: Log in to see
Antigen
  • EG667823
  • Gm8833
  • RGD1304579
  • RNF88
  • TRIM5
  • TRIM5alpha
Epitope
N-Term
41
20
15
13
9
6
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
133
6
2
1
1
1
Host
Goat
83
35
20
Clonality
Polyclonal
Conjugate
This TRIM5 antibody is un-conjugated
5
5
5
5
5
5
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
118
92
53
24
7
6
1
1
1
1
Supplier
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Immunogen Peptide with sequence ASGILVNVKEEVTC, from the N Terminus of the protein sequence according to NP_149023.1, NP_149083.1, NP_149084.1.
Blocking Peptide Blocking peptide for this product available: ABIN368761
Specificity This antibody is expected to recognise all three human isoforms of this protein.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TRIM5 / RNF88 (TRIM5 Antibody Abstract)
Background Synonyms: TRIM5, RNF88, tripartite motif-containing 5, TRIM5alpha, tripartite motif protein 5, tripartite motif protein TRIM5, OTTHUMP00000069812, tripartite motif protein TRIM, tripartite motif-containing protein 5 alpha
Gene ID 85363
NCBI Accession NP_149083, NP_149084, NP_149023
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 38 kDa band observed in Human Plancenta and Human Uterus lysates (calculated MW of 37.7 kDa according to NP_149084.1). Recommended concentration: 2-6 µg/mL.
IHC: A previous batch was successfully used on Human Tonsil.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-TRIM5 antibody (Tripartite Motif Containing 5) (N-Term) (ABIN184983) ABIN184983 staining (1 ug/ml) of Human Brain lysate (RIPA buffer, 35 ug total protein...
Immunohistochemistry (IHC) image for anti-TRIM5 antibody (Tripartite Motif Containing 5) (N-Term) (ABIN184983) ABIN184983 (1 ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen r...
Background publications Reymond, Meroni, Fantozzi, Merla, Cairo, Luzi, Riganelli, Zanaria, Messali, Cainarca, Guffanti, Minucci, Pelicci, Ballabio: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001