You are viewing an incomplete version of our website. Please click to reload the website as full version.

IFIH1 antibody (Interferon Induced with Helicase C Domain 1) (N-Term)

Details for Product anti-IFIH1 Antibody No. ABIN185002, Supplier: Log in to see
Antigen
  • 9130009C22Rik
  • Helicard
  • Hlcd
  • IDDM19
  • MDA-5
  • MDA5
  • RGD1560496
  • RLR-2
Epitope
N-Term
19
19
11
8
6
4
4
3
3
1
1
1
1
1
1
Reactivity
Human
115
46
4
2
1
Host
Goat
77
32
24
Clonality
Polyclonal
Conjugate
This IFIH1 antibody is un-conjugated
3
2
2
2
2
2
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
93
62
47
23
11
10
7
3
3
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Peptide with sequence SNGYSTDENFRYL-C, from the N Terminus of the protein sequence according to NP_071451.2.
Blocking Peptide Blocking peptide for this product available: ABIN370461
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MDA5 / IFIH1 (IFIH1 Antibody Abstract)
Background Synonyms: IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1, helicard
Gene ID 64135
NCBI Accession NP_071451
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: This antibody has been successfully used in transfected cells as described in the following paper: Sato et al, Arthritis Rheum. 2009 Jul,60(7):2193-200, PMID: 19565506.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-IFIH1 antibody (Interferon Induced with Helicase C Domain 1) (N-Term) (ABIN185002) ABIN185002 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen...
Product cited in: Sato, Hoshino, Satoh, Fujita, Kawakami, Fujita, Kuwana et al.: "RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial ..." in: Arthritis and rheumatism, Vol. 60, Issue 7, pp. 2193-200, 2009 (PubMed).

Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." in: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, 2008 (PubMed).

Bao, Sinha, Liu, Hong, Luxon, Garofalo, Casola: "Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis." in: Virology, Vol. 374, Issue 1, pp. 114-27, 2008 (PubMed).

Background publications Kang, Gopalkrishnan, Wu, Jankowsky, Pyle, Fisher: "mda-5: An interferon-inducible putative RNA helicase with double-stranded RNA-dependent ATPase activity and melanoma growth-suppressive properties." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 637-42, 2002 (PubMed).