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Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody

Details for Product No. ABIN185061, Supplier: Log in to see
Antigen
  • zfyve20
  • Rabenosyn-5
  • 5330426D11Rik
Epitope
C-Term
12
5
3
2
2
1
Reactivity
Human
27
11
3
3
2
2
Host
Goat
14
14
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
26
19
2
Supplier
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Immunogen Peptide with sequence C-ELKHTLAKQKGGTD, from the C Terminus of the protein sequence according to NP_071735.2.
Blocking Peptide Blocking peptide for this product available: ABIN369019
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Rabenosyn 5 (ZFYVE20 Antibody Abstract)
Background Synonyms: ZFYVE20, Rabenosyn 5, FYVE-finger-containing Rab5 effector protein rabenosyn-5, zinc finger, FYVE domain containing 20, zinc finger, FYVE domain containing 20, FLJ34993, MGC126210, Rabenosyn-5
Gene ID 64145
NCBI Accession NP_071735
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 110 kDa band observed in A431, Fibroblast (epidermoid carcinoma) lysates (calculated MW of 88.8 kDa according to NP_071735). Band migration is consistent with literature (PMID 11062261, 15020713). Recommended concentration: 0.3 - 1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Zinc Finger, FYVE Domain Containing 20 (ZFYVE20) (C-Term) antibody (ABIN185061) ABIN185061 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary ...
Background publications Nielsen, Christoforidis, Uttenweiler-Joseph et al.: "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 601-12, 2000 (PubMed).