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SMARCE1 antibody (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1) (C-Term)

Details for Product anti-SMARCE1 Antibody No. ABIN185062, Supplier: Log in to see
Antigen
  • BAF57
  • XBaf57
  • MGC52696
  • MGC69429
  • MGC81726
  • SMARCE1
  • DKFZp459O2235
  • fb54d11
  • wu:fb33d02
  • wu:fb54d11
  • Baf57
  • 2810417B20Rik
  • 5830412H02Rik
  • 9030408N19Rik
  • Baf27
  • RGD1304726
Epitope
C-Term
39
15
4
4
2
2
2
2
1
1
1
Reactivity
Human
79
15
10
4
2
2
2
1
1
1
1
Host
Goat
49
18
12
Clonality
Polyclonal
Conjugate
This SMARCE1 antibody is un-conjugated
3
3
3
3
3
3
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
67
40
38
12
12
11
5
2
1
1
Supplier
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Immunogen Peptide with sequence C-PPTDPIPEDEKKE, from the C Terminus of the protein sequence according to NP_003070.3.
Blocking Peptide Blocking peptide for this product available: ABIN370324
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BAF57 / SMARCE1 (SMARCE1 Antibody Abstract)
Background Synonyms: SMARCE1, BAF57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, mammalian chromatin remodeling complex BRG1-associated factor 57, BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
Gene ID 6605, 57376
NCBI Accession NP_003070
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 50 kDa band observed in lysates of cell lines HEPG2, HeLa and Jurkat (calculated MW of 46.6 kDa according to NP_003070.3). A 48 kDa band was observed in transfected HEK293 transiently expressing BAF57. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6 µg/mL. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EB06054 (mock transfection in first lane), tested by Origene. B) EB06054 staining (0.2 µg/mL) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IHC: In paraffin embedded Human Prostate shows nuclear staining in secretory epithelial cells. Recommended concentration: 3-5 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Wang, Chi, Xue et al.: "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 492-8, 1998 (PubMed).

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