Mastermind-Like 1 (Drosophila) (MAML1) (N-Term) antibody

Details for Product No. ABIN185074
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Antigen
Synonyms mam1, mam-1, xmam1, MAML1, AI644666, AW743257, D930008C07Rik, Mam-1, Mam1, mKIAA0200
Epitope
N-Term
(8), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(34), (14), (12), (12)
Host
Goat
(27), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(22), (14), (10), (10), (8), (4), (3), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185074
374.27 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Immunogen Peptide with sequence VLPTCPMAEFALPR, from the N Terminus of the protein sequence according to NP_055572.1.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name MAML1 / Mastermind
Background Synonyms: MAML1, mastermind-like 1 (Drosophila), Mam1, Mam-1, KIAA0200, mastermind homolog, mastermind-like 1
Gene ID 9794
NCBI Accession NP_055572
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 110 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 108 kDa according to NP_055572.1). Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration: 2.5-3.8 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Mastermind-Like 1 (Drosophila) (MAML1) (N-Term) antibody ABIN185074 (0.1µg/ml) staining of Human Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Wu, Aster, Blacklow et al.: "MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors." in: Nature genetics, Vol. 26, Issue 4, pp. 484-9, 2000 (PubMed).

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