KALRN antibody (C-Term)

Catalog No. ABIN185110

Product Details anti-KALRN Antibody

Target: KALRN (Kalirin, RhoGEF Kinase (KALRN))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KALRN antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: Kalirin

Sequence: RWHLGPGDPF STYV

Specificity: This antibody is expected to recognise NP_003938.1,(isoform 2),NP_001309918..1 (9isoform 5), NP_001309919.1,(isoform 6), NP_001309920.1 (isoform 7), and NP_001309921.1 (isoform 8).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RWHLGPGDPFSTYV, from the C Terminus of the protein sequence according to NP_003938.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4-5 μg/mL.

Western Blot: Preliminary testing showed a band at approx.. 200 kDa in Rat Brain lysate, after 0.1 μg/mL antibody staining (calculated Mwt. of 192.5 kDa according to XP_038944588.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KALRN

Target: KALRN (Kalirin, RhoGEF Kinase (KALRN))

Alternative Name: KALRN (KALRN Products)

Synonyms: ARHGEF24 antibody, CHD5 antibody, CHDS5 antibody, DUET antibody, DUO antibody, HAPIP antibody, TRAD antibody, KALRN antibody, 2210407G14Rik antibody, AV235988 antibody, E530005C20Rik antibody, Gm539 antibody, Hapip antibody, Duo antibody, Kalirin antibody, kalirin RhoGEF kinase antibody, kalirin, RhoGEF kinase antibody, kalirin antibody, kalirin RhoGEF kinase L homeolog antibody, putative kalirin antibody, KALRN antibody, LOC460643 antibody, Kalrn antibody, kalrn antibody, kalrn.L antibody, LOC100473997 antibody, Smp_162970 antibody

Background: KALRN, kalirin, RhoGEF kinase, FLJ16443, HAPIP, TRAD, duo, huntingtin-associated protein interacting protein (duo), serine/threonine kinase with Dbl- and pleckstrin homology domains, hCG_2022551, DUET, DUO, FLJ12332, FLJ18196, FLJ18623,

Molecular Weight: 192kDa

Gene ID: 8997, 545156, 84009

NCBI Accession: NP_003938

Pathways: Neurotrophin Signaling Pathway