Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1) antibody

Antigen: Split Hand/foot Malformation (Ectrodactyly) Type 1 (SHFM1)

Synonyms: ECD, DSS1, SEM1, SHFD1, SHSF1, Shfdg1, SHFM1, MGC128587

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185118

Additional Information

Alternative name: DSS1 / SHFM1

Gene ID: 7979, 20422 (mouse)

Immunogen: C-RAELEKHGYKMETS

Description: All names and symbols: SHFM1, split hand/foot malformation (ectrodactyly) type 1, DSS1, Deleted in split-hand/split-foot 1 region, ECD, SEM1, SHFD1, SHSF1, Shfdg1, candidate for split hand/foot malformation type 1, deleted in split-hand/foot 1, deleted in split-hand/split-foot 1. Official Symbol: SHFM1. RefSeq number(s): NP_006295.1. Gene ontology terms: molecular_function unknown, biological_process unknown, cellular_component unknown

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Liver extract at up to 1µg/ml. This used our standard western blotting protocol which we would not expect to detect proteins as small as the predicted size of approx. 8KDa according to NP_006295. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Wei, Trempus, Cannon et al.: "Identification of Dss1 as a 12-O-tetradecanoylphorbol-13-acetate-responsive gene expressed in keratinocyte progenitor cells, with possible involvement in early skin tumorigenesis." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 1758-68, 2003 (PubMed).