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Pleckstrin Homology-Like Domain, Family A, Member 3 (PHLDA3) antibody

Details for Product No. ABIN185126, Supplier: Log in to see
Antigen
  • TIH1
  • Tih1
  • si:dz182n13.5
  • zgc:92333
Reactivity
Human, Mouse (Murine)
27
12
12
Host
Goat
14
12
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
ELISA
19
18
8
1
1
Supplier
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Immunogen C-RARQSLGTGTLVS
Blocking Peptide Blocking peptide for this product available: ABIN369739
Specificity Human, Mouse
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PHLDA3 / TIH1 (PHLDA3 Antibody Abstract)
Background All names and symbols: PHLDA3, TIH1, pleckstrin homology-like domain, family A, member 3, pleckstrin homology-like domain, family A, member 2. Official Symbol: PHLDA3. RefSeq number(s): NP_036528. Gene ontology terms: embryogenesis and morphogenesis
Gene ID 23612, 27280
Application Notes Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 70kDa band in Human Placenta lysate after 1µg/ml antibody staining. This band was successfully blocked by the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 13.9kDa according to NP_036528. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Restrictions For Research Use only
Format Liquid
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Frank, Mendelsohn, Ciccone et al.: "A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 10, Issue 12, pp. 1150-9, 2000 (PubMed).