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Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) (C-Term) antibody

Details for Product No. ABIN185152, Supplier: Log in to see
Antigen
  • PARD6A
  • 0710008C04Rik
  • 2610010A15Rik
  • PAR6alpha
  • Par-6
  • Par6
  • Par6c
  • TAX40
  • Tip-40
  • Par-6a
  • Par6a
  • PAR-6A
  • PAR6
  • PAR6C
  • TIP-40
Epitope
C-Term
21
6
5
4
1
1
1
1
Reactivity
Human
54
20
18
6
1
1
1
1
Host
Goat
45
15
3
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
33
24
22
11
9
3
2
Supplier
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Immunogen Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1, NP_001032358.1.
Blocking Peptide Blocking peptide for this product available: ABIN369139
Specificity This antibody is expected to recognise both reported isoforms (NP_058644.1, NP_001032358.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PAR6alpha / PARD6A (PARD6A Antibody Abstract)
Background Synonyms: PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha, PAR6, PAR6C, Tax-interacting protein 40, par-6 partitioning defective 6 homolog alpha, partitioning defective-6 homolog alpha, partitioning-defective protein 6
Gene ID 50855
NCBI Accession NP_058644, NP_001032358
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1 µg/mL.
IHC: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10 µg/mL. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) (C-Term) antibody (ABIN185152) ABIN185152 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen...
Immunohistochemistry (IHC) image for anti-Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) (C-Term) antibody (ABIN185152) ABIN185152 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrie...
Background publications Johansson, Driessens, Aspenström: "The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1." in: Journal of cell science, Vol. 113 ( Pt 18), pp. 3267-75, 2000 (PubMed).