Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1) antibody

Antigen: Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1 (EIF4ENIF1)

Synonyms: 4E-T, Clast4, FLJ21601, FLJ26551, AA410001, AU021239, D11Ertd166e, 2610509L04Rik, A930019J01Rik, RGD1560908, eif4enif1, MGC80355, EIF4ENIF1, wu:fd15a05, zgc:101646, MGC147579

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185154

Additional Information

Alternative name: 4E-T / EIF4ENIF1

Gene ID: 56478, 74203 (mouse)

Immunogen: C-AKVISVDELEYRQ

Description: All names and symbols: EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter. Official Symbol: EIF4ENIF1. RefSeq number(s): NP_062817.1. Gene ontology terms: protein transporter, nucleocytoplasmic transport, cytoplasm, nucleus

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 140-150kDa band observed in 293 lysates (predicted size of approx. 108kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5µg/ml Immunohistochemistry: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185154 staining (0.25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN185154 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Dostie, Ferraiuolo, Pause et al.: "A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E." in: The EMBO journal, Vol. 19, Issue 12, pp. 3142-56, 2000 (PubMed).