KPNA2 antibody (C-Term)

Catalog No. ABIN185157

Product Details anti-KPNA2 Antibody

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Binding Specificity: C-Term

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: KPNA2 / IPOA1

Sequence: QVQDGAPGTF NF

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QVQDGAPGTFNF, from the C Terminus of the protein sequence according to NP_002257.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 5 μg/mL.

Western Blot: Approx 55 kDa band observed in lysates of cell lines CaCo-2, Jurkat, and KNRK, and approx. 58 kDa in lysates of cell lines MCF7 and A549 (calculated MW of 57.9 kDa according to Human NP_002257.1 and Rat NP_001257731.1). Recommended concentra

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in U2OS and A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KPNA2

Target: KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))

Alternative Name: KPNA2 (KPNA2 Products)

Synonyms: IPOA1 antibody, QIP2 antibody, RCH1 antibody, SRP1alpha antibody, ima2 antibody, importin antibody, 2410044B12Rik antibody, PTAC58 antibody, Rch1 antibody, ipoa1 antibody, kpna2 antibody, qip2 antibody, rch1 antibody, srp1alpha antibody, hm:zeh0389 antibody, hm:zeh0389r antibody, zgc:113902 antibody, zgc:86945 antibody, karyopherin subunit alpha 2 antibody, importin subunit alpha-2 antibody, Importin subunit alpha-2 antibody, karyopherin (importin) alpha 2 antibody, karyopherin alpha-2 subunit like L homeolog antibody, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) antibody, KPNA2 antibody, EDI_246290 antibody, EDI_335040 antibody, ima2 antibody, Kpna2 antibody, kpna2.L antibody, kpna2 antibody

Background: KPNA2, IPOA1 QIP2, RCH1, SRP1alpha, karyopherin alpha 2 (RAG cohort 1, importin alpha 1), RAG cohort 1, importin alpha 1, importin alpha 2, importin-alpha-P1, karyopherin alpha 2, pendulin

Gene ID: 3838, 16647, 85245

NCBI Accession: NP_002257

Pathways: M Phase, Protein targeting to Nucleus