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VPS35 antibody (C-Term)

Verified VPS35 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN185182
  • Target See all VPS35 Antibodies
    VPS35 (Vacuolar Protein Sorting 35 (VPS35))
    Binding Specificity
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 96
    • 22
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 58
    • 38
    Goat
    Clonality
    • 68
    • 28
    Polyclonal
    Conjugate
    • 25
    • 8
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This VPS35 antibody is un-conjugated
    Application
    • 74
    • 57
    • 48
    • 47
    • 38
    • 7
    • 5
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA
    Purpose
    VPS35 / MEM3
    Sequence
    SPESEGPIYE GLIL
    Specificity
    Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

    Western Blot: Approx 90 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85 kDa observed in lysates of cell line HEK293 (calculated MW of 91.7 kDa according to Human NP_060676.2, Mouse NP_0

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Klinger, Højland, Jain, Kjolby, Madsen, Svendsen, Olivecrona, Bonifacino, Nielsen: "Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells." in: The FEBS journal, Vol. 283, Issue 13, pp. 2476-93, (2017) (PubMed).

    Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, (2012) (PubMed).

  • Target
    VPS35 (Vacuolar Protein Sorting 35 (VPS35))
    Alternative Name
    VPS35 (VPS35 Products)
    Synonyms
    MEM3 antibody, PARK17 antibody, vps35 antibody, MGC81959 antibody, bZ34G2.3 antibody, si:rp71-34g2.3 antibody, wu:fa66e03 antibody, wu:fb95c08 antibody, zgc:136268 antibody, 23.m05999 antibody, Mem3 antibody, VPS35 antibody, DKFZp459E1510 antibody, CG5625 antibody, DVps35 antibody, Dmel\\CG5625 antibody, Dvps35 antibody, dvps35 antibody, AI647796 antibody, mem3 antibody, VPS35, retromer complex component antibody, VPS35 retromer complex component L homeolog antibody, VPS35 retromer complex component antibody, vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, vacuolar protein sorting-associated protein 35 antibody, vacuolar protein sorting 35 antibody, Vacuolar protein sorting 35 antibody, hypothetical protein antibody, VPS35 retromer complex component S homeolog antibody, retromer complex subunit Vps35 antibody, VPS35 antibody, vps35.L antibody, vps35 antibody, Tc00.1047053511367.170 antibody, Tb10.70.5460 antibody, BBOV_IV009740 antibody, CC1G_12185 antibody, PTRG_08682 antibody, CMU_017400 antibody, PHATR_43830 antibody, MCYG_00751 antibody, MGYG_01212 antibody, EHI_086580 antibody, EHI_002990 antibody, EHI_041950 antibody, EHI_096520 antibody, Bm1_44045 antibody, LOC100285267 antibody, Vps35 antibody, PGTG_10018 antibody, Tsp_09825 antibody, vps35.S antibody
    Background
    VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35
    Gene ID
    55737, 65114
    NCBI Accession
    NP_060676
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