BAF53A and BAF53B (C-Term) antibody

Catalog No. ABIN185217

Product Details

Target: BAF53A and BAF53B

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: BAF53A and BAF53B

Sequence: YEEGGKQCVE RKCP

Specificity: This antibody is expected to recognise BAF53A isoforms 1 and 2 and BAF53B, which are almost identical

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence YEEGGKQCVERKCP, from the C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in Rat Skeletal Muscle lysates and approx. 50 kDa in nuclear lysates of cell line NIH3T3 and in preliminary testing of Human Colorectal cancer and Mouse Skeletal Muscle lysate.(predicted MW of 47.4 kDa (Actl6a) acco

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: BAF53A and BAF53B

Alternative Name: ACTL6A / ACTL6B

Synonyms: ACTL6 antibody, BAF53B antibody, Actl6 antibody, ArpNa antibody, Baf53b antibody, actin like 6B antibody, actin-like 6B antibody, ACTL6B antibody, Actl6b antibody

Background: BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B, 53 kDa BRG1-associated factor B, ARPN-BETA, MGC5382 Arp4, INO80K, BAF complex 53 kDa subunit, BAF53, BRG1-associated factor, INO80 com

Gene ID: 51412, 86

NCBI Accession: NP_004292, NP_817126, NP_057272