Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (C-Term) antibody

Details for Product No. ABIN185242
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Antigen
Synonyms AW556805, Mcl-1, BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S, TM, bcl2-L-3, mcl1/EAT, mcl-1
Epitope
C-Term
(19), (18), (17), (15), (12), (9), (7), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(212), (83), (60), (18), (12), (3), (3), (2), (2), (1)
Host
Goat
(163), (46), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(171), (86), (66), (46), (38), (31), (21), (20), (13), (11), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185242
374.27 $
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Immunogen Peptide with sequence C-AGVGAGLAYLIR, from the C Terminus of the protein sequence according to NP_068779.1, NP_001184249.1.
Specificity This antibody is expected to recognise reported isoform 1 (NP_068779.1) and isoform 3 (NP_001184249.1).
Cross-Reactivity Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MCL1
Background Synonyms: MCL1, TM, EAT, MCL1L, MCL1S, MGC1839, MGC104264, myeloid cell leukemia sequence 1 (BCL2-related), induced myeloid leukemia cell differentiation protein Mcl-1, myeloid cell leukemia sequence 1, myeloid cell leukemia sequence 1, isoform 1, BCL2L3, Mcl-1
Gene ID 4170, 17210
NCBI Accession NP_068779, NP_001184249
Application Notes ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 35 kDa band observed in lysates of Human Lung, in Mouse and Rat Thymus, and in cell line K562 (calculated MW of 37.3 kDa according to NP_068779.1). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (C-Term) antibody ABIN185242 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Opferman, Letai, Beard et al.: "Development and maintenance of B and T lymphocytes requires antiapoptotic MCL-1." in: Nature, Vol. 426, Issue 6967, pp. 671-6, 2003 (PubMed).

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