Bcl-2 antibody (N-Term)
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- Target See all Bcl-2 (BCL2) Antibodies
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Bcl-2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Purpose
- BCL2
- Sequence
- AGRTGYDNRE IVMKYC
- Specificity
- This antibody is expected to recognise both reported isoforms (NP_000624, NP_000648)
- Cross-Reactivity
- Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence AGRTGYDNREIVMKYC, from the N Terminus of the protein sequence according to NP_000624.2, NP_000648.2.
- Isotype
- IgG
- Top Product
- Discover our top product BCL2 Primary Antibody
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- Application Notes
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Western Blot: Approx 27+25 kDa band observed in Human Lymph lysates (calculated MW of 26.3 kDa according to NP_000624.2 and of 22.2 kDa according to NP_000648.2). Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
- Alternative Name
- BCL2 (BCL2 Products)
- Synonyms
- Bcl-2 antibody, PPP1R50 antibody, bcl-2 antibody, AW986256 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, BCL-2 antibody, Bcl2 antibody, BCL2, apoptosis regulator antibody, B cell leukemia/lymphoma 2 antibody, B-cell CLL/lymphoma 2a antibody, B-cell CLL/lymphoma 2 protein antibody, BCL2 antibody, Bcl2 antibody, bcl2a antibody, BCL-2 antibody
- Background
- BCL2, Bcl-2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, OTTHUMP00000163680
- Gene ID
- 596, 12043, 24224
- NCBI Accession
- NP_000624, NP_000648
- Pathways
- MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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