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Survivin (BIRC5) (C-Term) antibody

Details for Product No. ABIN185270
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Antigen
Synonyms bir5a, survivin, LOC398389, BIRC5, BIR1, Su2, Survivin, Survivin1, XBIR1, api4, epr-1, svv1, xSurvivin1, xsurv, SIX, Su1, svv2, birc5, xSurvivin, xSurvivin2, AAC-11, Api4, TIAP, survivin40
Epitope
C-Term
(42), (17), (15), (8), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(218), (90), (75), (31), (24), (24), (12), (8), (1)
Host
Goat
(186), (50), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(12), (7), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(166), (73), (63), (60), (53), (52), (20), (16), (5), (5), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185270
374.27 $
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Immunogen Peptide with sequence CVRRAIEQLAAMD, from the C Terminus of the protein sequence according to NP_001159.2, NP_001012271.1.
Specificity This antibody is expected to recognize the reported isoforms 1 (NP_001159.2) and isoform 3 (NP_001012271.1).
Cross-Reactivity Cat (Feline), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Survivin / BIRC5
Background Synonyms: BIRC5, survivin, API4, EPR-1, baculoviral IAP repeat-containing 5 (survivin), apoptosis inhibitor 4, baculoviral IAP repeat-containing 5, baculoviral IAP repeat-containing protein 5, survivin variant 3 alpha
Gene ID 332
NCBI Accession NP_001159.2, NP_001012271.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 18 kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 16.4 kDa according to NP_001159.2 and 18.6 kDa according to NP_001012271.1). Recommended concentration: 0.01-0.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Survivin (BIRC5) (C-Term) antibody ABIN185270 (0.01µg/ml) staining of Molt-4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Li, Ambrosini, Chu et al.: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999 (PubMed).

Validation Images
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