Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) (C-Term) antibody

Details for Product No. ABIN185275
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Antigen
Synonyms dkk, dkk-1, xdkk1, Xdkk-1, Dickkopf1, dickkopf-1, dkk1, mdkk-1, DKK-1, SK, dkk1-A, CT34, SGY, SGY-1, SGY1, AI414495, Soggy, mSgy
Epitope
C-Term
(13), (13), (10), (8), (8), (6), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(116), (29), (25), (3), (2), (2), (1), (1), (1), (1)
Host
Goat
(80), (32), (8), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(90), (53), (27), (10), (10), (4), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185275
374.27 $
Plus shipping costs $45.00

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  • +1 404 474 4654
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Immunogen Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.1.
Cross-Reactivity Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DKK1
Background Synonyms: DKK1, SK, DKK-1, dickkopf homolog 1 (Xenopus laevis), dickkopf-1 like, dickkopf (Xenopus laevis) homolog 1, dickkopf homolog 1, dickkopf related protein-1
Gene ID 22943
NCBI Accession NP_036374
Research Area Embryogenesis, Wnt
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 38 kDa band observed in Human Spleen ysates (calculated MW of 28.7 kDa according to NP_036374.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fedi et al, J Biol Chem. 1999 Jul 2,274(27):19465-72, PMID: 10383463). Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Dickkopf Homolog 1 (Xenopus Laevis) (DKK1) (C-Term) antibody ABIN185275 (1µg/ml) staining of human bone marrow lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Fedi, Bafico, Nieto Soria et al.: "Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling." in: The Journal of biological chemistry, Vol. 274, Issue 27, pp. 19465-72, 1999 (PubMed).

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