Vav 3 Oncogene (VAV3) (Internal Region) antibody

Details for Product No. ABIN185278
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Antigen
Synonyms RGD1565941, A530094I06Rik, AA986410, Idd18.1
Epitope
Internal Region
(21), (13), (12), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(72), (55), (36), (24), (13)
Host
Goat
(67), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(46), (30), (20), (14), (6), (4), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN185278
374.27 $
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Immunogen Peptide with sequence CSGEQGTLKLPEK, from the internal region of the protein sequence according to NP_006104.4, NP_001073343.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_006104.4, NP_001073343.1).
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name VAV3
Background Synonyms: VAV3, vav 3 oncogene, vav 3 guanine nucleotide exchange factor, FLJ40431
Gene ID 10451
NCBI Accession NP_001073343, NP_006104
Research Area Cancer, Cell Cycle
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 90 kDa band observed in human bone marrow lysates (calculated MW of 97.8 kDa according to NP_006104). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Vav 3 Oncogene (VAV3) (Internal Region) antibody ABIN185278 (0.3 ug/ml) staining of human bone marrow lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Vav 3 Oncogene (VAV3) (Internal Region) antibody (2) ABIN185278 (1 µg/mL) staining of human bone marrow lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Movilla, Bustelo: "Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins." in: Molecular and cellular biology, Vol. 19, Issue 11, pp. 7870-85, 1999 (PubMed).

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