HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2) antibody

Antigen: HECT, C2 and WW Domain Containing E3 Ubiquitin Protein Ligase 2 (HECW2)

Synonyms: NEDL2, DKFZp686M17164, Gm971, Nedl2, mKIAA1301, A730039N16Rik, D030049F17Rik, HECW2

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN185281

Additional Information

Alternative name: EndoNEDD4 / NEDL2

Gene ID: 57520, 227106 (mouse)

Immunogen: ASSAREHLLFVRR-C

Description: All names and symbols: HECW2, HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2, NEDD4-related E3 ubiquitin ligase NEDL2, KIAA1301 protein, EndoNEDD4. Official Symbol: HECW2. RefSeq number(s): NP_065811.1. Gene ontology terms: ubiquitin-protein ligase activity, ubiquitin cycle, protein modification, intracellular

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Lung and Kidney lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Nagase, Kikuno, Ishikawa et al.: "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 1, pp. 65-73, 2000 (PubMed).