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Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region)

Details for Product anti-CASP4 Antibody No. ABIN185284, Supplier: Log in to see
Antigen
  • CASP4
  • ICE(rel)II
  • ICEREL-II
  • ICH-2
  • Mih1/TX
  • TX
  • Casp11
  • Caspl
  • ich-3
  • CASP1
  • CASP13
Alternatives
anti-Human Caspase 4 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
Internal Region
42
25
14
10
6
5
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
163
73
30
20
2
1
1
Host
Goat
181
21
14
12
6
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
13
11
3
2
2
2
2
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
189
84
52
44
12
12
11
8
8
5
3
3
2
1
1
Supplier
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Immunogen Peptide with sequence CKERAEEIYPIKERN, from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.
Blocking Peptide Blocking peptide for this product available: ABIN369266
Specificity This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).
Cross-Reactivity Mouse (Murine), Cat (Feline), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CASP4 (CASP4 Antibody Abstract)
Background Synonyms: CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX, caspase 4
Gene ID 837
NCBI Accession NP_001216, NP_150649
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in Human Heart lysates (calculated MW of 43.3 kDa according to NP_001216.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region) (ABIN185284) ABIN185284 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). P...
 image for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region) (ABIN185284) ABIN185284 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Pri...
Background publications Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).