Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) (Internal Region) antibody

Details for Product No. ABIN185284
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Antigen
Synonyms TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, Casp11, Caspl, ich-3, CASP4
Epitope
Internal Region
(24), (11), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(106), (62), (33), (12), (7), (2)
Host
Goat
(121), (12), (8), (5), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(128), (56), (44), (34), (19), (18), (15), (6), (3), (2), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN185284
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
Options
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Immunogen Peptide with sequence CKERAEEIYPIKERN, from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.
Specificity This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).
Cross-Reactivity Mouse (Murine), Cat (Feline), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CASP4
Background Synonyms: CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX, caspase 4
Gene ID 837
NCBI Accession NP_001216.1, NP_150649.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in Human Heart lysates (calculated MW of 43.3 kDa according to NP_001216.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) (Internal Region) antibody ABIN185284 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) (Internal Region) antibody (2) ABIN185284 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).

Hosts (121), (12), (8), (5), (4)
Reactivities (106), (62), (33), (12), (7), (2)
Applications (128), (56), (44), (34), (19), (18), (15), (6), (3), (2), (1), (1)
Conjugates (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (24), (11), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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