anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region)

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Antigen
Synonyms CASP4, ICE(rel)II, ICEREL-II, ICH-2, Mih1/TX, TX, Casp11, Caspl, ich-3, CASP1, CASP13
Alternatives
Epitope
Internal Region
(43), (28), (16), (12), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(174), (85), (34), (23), (3), (1), (1)
Host
Goat
(196), (23), (16), (15), (7)
Clonality
Polyclonal
Conjugate
This Caspase 4 antibody is un-conjugated
(14), (11), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(211), (98), (55), (54), (20), (20), (12), (10), (8), (6), (3), (3), (2), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence CKERAEEIYPIKERN, from the internal region of the protein sequence according to NP_001216.1, NP_150649.1.
Blocking Peptide Blocking peptide for this product available: Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4) peptide (ABIN369266)
Specificity This antibody is expected to recognise both isoforms of CASP4 (alpha, represented by NP_001216.1 and gamma, NP_150649.1).
Cross-Reactivity Mouse (Murine), Cat (Feline), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CASP4 (CASP4 Antibody Abstract)
Background Synonyms: CASP4, TX, ICH-2, Mih1/TX, ICEREL-II, ICE(rel)II, caspase 4, apoptosis-related cysteine protease, apoptotic cysteine protease Mih1/TX, caspase 4
Gene ID 837
NCBI Accession NP_001216, NP_150649
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 40 kDa band observed in Human Heart lysates (calculated MW of 43.3 kDa according to NP_001216.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Image no. 1 for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region) (ABIN185284) ABIN185284 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). P...
Image no. 2 for anti-Caspase 4 antibody (Caspase 4, Apoptosis-Related Cysteine Peptidase) (Internal Region) (ABIN185284) ABIN185284 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer). Pri...
Background publications Munday, Vaillancourt, Ali et al.: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).

Catalog No. ABIN185284
$221.76
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