Retinoid X Receptor alpha antibody (AA 14-28)

Catalog No. ABIN185295

Product Details anti-Retinoid X Receptor alpha Antibody

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Binding Specificity: AA 14-28

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Retinoid X Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: RXR alpha

Sequence: CQVNSSLTSP TGRGSM

Specificity: This antibody is expected to recognise an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CQVNSSLTSPTGRGSM, from the internal region of the protein sequence according to NP_002948.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell lines HeLa and K562 (calculated MW of 50.8 kDa according to NP_002948.1). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Retinoid X Receptor alpha

Target: Retinoid X Receptor alpha (RXRA) (Retinoid X Receptor, alpha (RXRA))

Alternative Name: RXRA (RXRA Products)

Synonyms: NR2B1 antibody, 9530071D11Rik antibody, Nr2b1 antibody, RXRalpha1 antibody, RXRalpha antibody, rxra-A antibody, xRXR alpha antibody, xrxra antibody, RXRA antibody, RXR alpha antibody, etID309731.5 antibody, rxr antibody, rxra antibody, rxrg antibody, RXRalpha-B antibody, retinoid X receptor alpha antibody, retinoid X receptor alpha L homeolog antibody, retinoid x receptor, alpha b antibody, retinoid X receptor, alpha a antibody, RXRA antibody, Rxra antibody, rxra.L antibody, CpipJ_CPIJ010249 antibody, rxrab antibody, rxraa antibody

Background: RXRA, NR2B1, retinoid X receptor, alpha, FLJ16020, FLJ16733, MGC102720, FLJ00280, FLJ00318,

Gene ID: 6256

NCBI Accession: NP_002948

Pathways: Nuclear Receptor Transcription Pathway, Retinoic Acid Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Hepatitis C